BSU_00290

BSU00290

BSU_00290

BSU00290

MGNA-B897 (yaaQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1896 NBRP B. subtilis, Japan]

GP1712 (''[[gene|darA]]''::''cat''), available in [SW|Jörg Stülke]'s lab [Pubmed|25433025]

GP1713 (''[[gene|darA]]-[[gene|yaaR]]''::''cat''), available in [SW|Jörg Stülke]'s lab

GP2406 (''[[gene|darA]]''::''ermC''), available in [SW|Jörg Stülke]'s lab

GP2415 (''[[gene|darA]]''::''spc''), available in [SW|Jörg Stülke]'s lab

GP2497 (''[[gene|darA]]''::''tet''), available in [SW|Jörg Stülke]'s lab

BKE00290 ([[gene|darA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGAAGGCCTCCTTT, downstream forward: _UP4_CATCAATTTTAAGGATCTGA

BKK00290 ([[gene|darA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGAAGGCCTCCTTT, downstream forward: _UP4_CATCAATTTTAAGGATCTGA

MGNA-B897 (yaaQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1896 NBRP B. subtilis, Japan]

GP1712 (''[[gene|darA]]''::''cat''), available in [SW|Jrg Stlke]'s lab [Pubmed|25433025]

GP1713 (''[[gene|darA]]-[[gene|yaaR]]''::''cat''), available in [SW|Jrg Stlke]'s lab

GP2406 (''[[gene|darA]]''::''ermC''), available in [SW|Jrg Stlke]'s lab

GP2415 (''[[gene|darA]]''::''spc''), available in [SW|Jrg Stlke]'s lab

GP2497 (''[[gene|darA]]''::''tet''), available in [SW|Jrg Stlke]'s lab

BKE00290 ([[gene|darA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGAAGGCCTCCTTT, downstream forward: _UP4_CATCAATTTTAAGGATCTGA

BKK00290 ([[gene|darA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGAAGGCCTCCTTT, downstream forward: _UP4_CATCAATTTTAAGGATCTGA

pGP2797: expression of ''darA'' (native RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

pGP3002: expression of ''darA'' (''gapA'' RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab [Pubmed|25433025]

pGP1690: IPTG inducible expression, purification in ''E. coli'' with N-terminal cleavable His-tag, in [SW|pGP570], available in [SW|Jörg Stülke]'s lab

pGP2624: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP2602: expression of Strep-''darA'' by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP2603: expression of ''darA''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

GP2010: expression of ''darA''-Strep in ''B. subtilis'' (chromosomal), suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP2797: expression of ''darA'' (native RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jrg Stlke]'s lab

pGP3002: expression of ''darA'' (''gapA'' RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jrg Stlke]'s lab

pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jrg Stlke]'s lab [Pubmed|25433025]

pGP1690: IPTG inducible expression, purification in ''E. coli'' with N-terminal cleavable His-tag, in [SW|pGP570], available in [SW|Jrg Stlke]'s lab

pGP2624: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jrg Stlke]'s lab

pGP2602: expression of Strep-''darA'' by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jrg Stlke]'s lab

pGP2603: expression of ''darA''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jrg Stlke]'s lab

GP2010: expression of ''darA''-Strep in ''B. subtilis'' (chromosomal), suitable for [SW|SPINE], available in [SW|Jrg Stlke]'s lab

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab

available in [SW|Jörg Stülke]'s lab

available in [SW|Jrg Stlke]'s lab